Journal: Plant Physiology

Article Title: Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots ^{1 [C] [W]}

doi: 10.1104/pp.113.215194

Figure Lengend Snippet: NO production and redox status in Arabidopsis seedlings exposed to cadmium. A, Seven-day-old Arabidopsis wild-type seedlings treated with 0.1 mm cadmium for 24 and 72 h and stained with DAF-FM diacetate for NO quantification. The relative fluorescence values are related to the fluorescence level in control roots (set to 1). False-colored pictures are representative images of transition and elongation root tip zones considered for the data analyses reported in the graph of A. B, Normalized ratio values (405/488 nm) of cytosolic roGFP2 probe in 7-d-old Arabidopsis roGFP2 transgenic seedlings treated with 0.1 mm cadmium for 24 and 72 h. C, Normalized ratio values (405/488 nm) of cytosolic roGFP2 probe in 7-d-old Arabidopsis roGFP2 transgenic seedlings treated with 1 mm 3-AT for 24 and 72 h. False-colored pictures are representative ratiometric images (405/488-nm ratios) of transition and elongation root tip zones considered for the data analyses reported in the graphs of B and C. Asterisks indicate fluorescence levels or normalized ratios that are significantly different from those found in control roots as calculated by Student’s t test (***P < 0.005 and **P < 0.01).

Article Snippet: Detection of NO Production For detection of NO production, 7-d-old Arabidopsis seedlings were incubated with the cell-permeable fluorescent probe DAF-FM diacetate (Alexis Biochemicals), with a final concentration of 5 μ m in the loading buffer solution (0.25 m m KCl, 1 m m CaCl 2 , and 5 m m MES-KOH, pH 5.7) for 15 min. Seedlings were then washed three times with fresh buffer and examined by confocal microscope as previously reported ( Zottini et al., 2007 ).

Techniques: Staining, Fluorescence, Transgenic Assay

Journal: International immunopharmacology

Article Title: Interleukin-33 induces angiogenesis after myocardial infarction via AKT/eNOS signaling pathway.

doi: 10.1016/j.intimp.2024.113433

Figure Lengend Snippet: Fig. 5. IL-33 stimulates NO production via Akt/eNOS signaling pathway in ECs. A, Representative Western blot images of p-eNOS, eNOS, p-AKT and AKT levels in HUVECs. B and D, Western blot analysis and quantification of p-eNOS/eNOS and p-AKT/AKT expressed in HUVECs (representative Western blot from three independent biological replicates, n = 3). C, Representative fluorescence images of HUVECs stained with NO fluorescent probe (DAF-FM DA) under different conditions, scale bar = 20 μm. *p < 0.05, **p < 0.01, and ***p < 0.001, one-way ANOVA, Tukey’s multiple comparison test.

Article Snippet: The NO levels in HUVECs were assessed using a DAF-FM DA probe (HY-D0717; MedChemExpress, China).

Techniques: Western Blot, Fluorescence, Staining, Comparison